In vivo model for frontal sinus and calvarial bone defect obliteration with bioactive glass S53P4 and hydroxyapatite

AbstractTissue engineering is a promising strategy for bone tissue defect reconstruction. Immunogenic reaction, which was induced by scaffolds degradation or contaminating microorganism, influence cellular activity, compromise the efficiency of tissue engineering, or eventually lead to the failure of regeneration. Inhibiting excessive immune response through modulating scaffold is critical important to promote tissue regeneration. Our previous study showed that ε-poly-L-lysine (EPL)-coated nanoscale polycaprolactone/hydroxyapatite (EPL/PCL/HA) composite scaffold has enhanced antibacterial and osteogenic properties in vitro. However, the bone defect repair function and immunogenic reaction of EPL/PCL/HA scaffolds in vivo remains unclear. In the present study, three nanoscale scaffolds (EPL/PCL/HA, PCL and PCL/HA) were transplanted into rabbit paraspinal muscle pouches, and T helper type 1 (Th1), T helper type 2 (Th2), T helper type 17 (Th17), and macrophage infiltration were analyzed after 1 week and 2 weeks to detect their immunogenic reaction. Then, the different scaffolds were transplanted into rabbit calvarial bone defect to compare the bone defect repair capacities. The results showed that EPL/PCL/HA composite scaffolds decreased pro-inflammatory Th1, Th17, and type I macrophage infiltration from 1 to 2 weeks, and increased anti-inflammatory Th2 infiltration into the regenerated area at 2 weeks in vivo, when compared to PCL and PCL/HA. In addition, EPL/PCL/HA showed an enhanced bone repair capacity compared to PCL and PCL/HA when transplanted into rabbit calvarial bone defects at both 4 and 8 weeks. Hence, our results suggest that EPL could regulate the immunogenic reaction and promote bone defect repair function of PCL/HA, which is a promising agent for tissue engineering scaffold modulation.

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In Vitro Prevascularization of Self-Assembled Human Bone-Like Tissues and Preclinical Assessment Using a Rat Calvarial Bone Defect Model

Materials ◽

10.3390/ma14082023 ◽

2021 ◽

Vol 14 (8) ◽

pp. 2023

Author(s):

Fabien Kawecki ◽

Todd Galbraith ◽

William P. Clafshenkel ◽

Michel Fortin ◽

François A. Auger ◽

...

Keyword(s):

Bone Defect ◽

Bone Repair ◽

Human Bone ◽

Male Rats ◽

Defect Model ◽

Calvarial Bone ◽

Calvarial Bone Defect ◽

Self Assembled

In vitro prevascularization has the potential to address the challenge of maintaining cell viability at the core of engineered constructs, such as bone substitutes, and to improve the survival of tissue grafts by allowing quicker anastomosis to the host microvasculature. The self-assembly approach of tissue engineering allows the production of biomimetic bone-like tissue constructs including extracellular matrix and living human adipose-derived stromal/stem cells (hASCs) induced towards osteogenic differentiation. We hypothesized that the addition of endothelial cells could improve osteogenesis and biomineralization during the production of self-assembled human bone-like tissues using hASCs. Additionally, we postulated that these prevascularized constructs would consequently improve graft survival and bone repair of rat calvarial bone defects. This study shows that a dense capillary network spontaneously formed in vitro during tissue biofabrication after two weeks of maturation. Despite reductions in osteocalcin levels and hydroxyapatite formation in vitro in prevascularized bone-like tissues (35 days of culture), in vivo imaging of prevascularized constructs showed an improvement in cell survival without impeding bone healing after 12 weeks of implantation in a calvarial bone defect model (immunocompromised male rats), compared to their stromal counterparts. Globally, these findings establish our ability to engineer prevascularized bone-like tissues with improved functional properties.

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Regeneration of Bone Defects Using Bioactive Glass Combined with Adipose-derived Mesenchymal Stem Cells. An experimental in vivo study

Revista de Chimie ◽

10.37358/rc.19.6.7259 ◽

2019 ◽

Vol 70 (6) ◽

pp. 1983-1987

Author(s):

Cristian Trambitas ◽

Anca Maria Pop ◽

Alina Dia Trambitas Miron ◽

Dorin Constantin Dorobantu ◽

Flaviu Tabaran ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Bioactive Glass ◽

Bone Repair ◽

Bone Defects ◽

Calvarial Bone ◽

Specific Protocol ◽

Repair Mechanisms ◽

Male Wistar Rats

Large bone defects are a medical concern as these are often unable to heal spontaneously, based on the host bone repair mechanisms. In their treatment, bone tissue engineering techniques represent a promising approach by providing a guide for osseous regeneration. As bioactive glasses proved to have osteoconductive and osteoinductive properties, the aim of our study was to evaluate by histologic examination, the differences in the healing of critical-sized calvarial bone defects filled with bioactive glass combined with adipose-derived mesenchymal stem cells, compared to negative controls. We used 16 male Wistar rats subjected to a specific protocol based on which 2 calvarial bone defects were created in each animal, one was filled with Bon Alive S53P4 bioactive glass and adipose-derived stem cells and the other one was considered control. At intervals of one week during the following month, the animals were euthanized and the specimens from bone defects were histologically examined and compared. The results showed that this biomaterial was biocompatible and the first signs of osseous healing appeared in the third week. Bone Alive S53P4 bioactive glass could be an excellent bone substitute, reducing the need of bone grafts.

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Erratum to: Osteoconduction capacity of human deciduous and permanent teeth ash in a rat calvarial bone defect model

Cell and Tissue Banking ◽

10.1007/s10561-015-9509-6 ◽

2015 ◽

Vol 16 (4) ◽

pp. 651-651

Author(s):

Boram Min ◽

Je Seon Song ◽

Seong-Oh Kim ◽

Kwang-Mahn Kim ◽

Won Se Park ◽

...

Keyword(s):

Bone Defect ◽

Permanent Teeth ◽

Defect Model ◽

Calvarial Bone ◽

Calvarial Bone Defect

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A Versatile Protocol for Studying Calvarial Bone Defect Healing in a Mouse Model

Tissue Engineering Part C Methods ◽

10.1089/ten.tec.2017.0205 ◽

2017 ◽

Vol 23 (11) ◽

pp. 686-693 ◽

Cited By ~ 13

Author(s):

Rebekah M. Samsonraj ◽

Amel Dudakovic ◽

Pengfei Zan ◽

Oksana Pichurin ◽

Simon M. Cool ◽

...

Keyword(s):

Mouse Model ◽

Bone Defect ◽

Calvarial Bone ◽

Defect Healing ◽

Bone Defect Healing ◽

Calvarial Bone Defect

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Repair of Calvarial Bone Defect Using Jarid1a-Knockdown Bone Mesenchymal Stem Cells in Rats

Tissue Engineering Part A ◽

10.1089/ten.tea.2017.0168 ◽

2018 ◽

Vol 24 (9-10) ◽

pp. 711-718 ◽

Cited By ~ 8

Author(s):

Yuan Deng ◽

Tao Guo ◽

Jipeng Li ◽

Li Guo ◽

Ping Gu ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Bone Defect ◽

Calvarial Bone ◽

Bone Mesenchymal Stem Cells ◽

Calvarial Bone Defect

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Evaluating the Effect of Non-cellular Bioactive Glass-Containing Scaffolds on Osteogenesis and Angiogenesis in in vivo Animal Bone Defect Models

Frontiers in Bioengineering and Biotechnology ◽

10.3389/fbioe.2020.00430 ◽

2020 ◽

Vol 8 ◽

Author(s):

Chanuka D. S. Ranmuthu ◽

Charindu K. I. Ranmuthu ◽

Jodie C. Russell ◽

Disha Singhania ◽

Wasim S. Khan

Keyword(s):

Bioactive Glass ◽

Bone Defect ◽

Animal Bone

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Osteogenic Potential Using a Malleable, Biodegradable Composite Added Traditional Chinese Medicine: in vitro and in vivo Evaluations

The American Journal of Chinese Medicine ◽

10.1142/s0192415x06004351 ◽

2006 ◽

Vol 34 (05) ◽

pp. 873-886 ◽

Cited By ~ 6

Author(s):

Chun-Hsu Yao ◽

Bai-Shuan Liu ◽

Chau-Guey Liu ◽

Yueh-Sheng Chen

Keyword(s):

Bone Substitute ◽

Bone Growth ◽

In Vitro Study ◽

Osteogenic Potential ◽

Calvarial Bone ◽

Large Defect ◽

Biodegradable Composite ◽

Calvarial Bone Defect

The purpose of this investigation was to prepare and evaluate the feasibility and biocompatibility of a new composite as a large defect bone substitute. The new GTGG was mainly composed of tricalcium phosphate ceramic particles and glutaraldehyde crosslinked gelatin in which Gui-Lu-Jiao was added (a mixture of Cervi Colla Cornus and Colla Plastri Testudinis). In the in vitro study, rat's calvaria osteoblasts were used to study bone characteristics upon exposure to different concentrations of the Gui-Lu-Jiao solution. In the in vivo study, GTGG composites were implanted into the defects of calvarial bones in mature New Zealand rabbits to test their osteogenerative characteristics. As a result, we found that Gui-Lu-Jiao added to the culture could promote the proliferation of osteoblasts. In addition, GTGG could induce a large amount of new bone growth in the rabbit's calvarial bone defect. Therefore, the GTGG composite might be a potential bone substitute.

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